![]() ![]() ![]() Assessing four subdomain-specific affinity resins' capability to separate half-antibody from intact bispecific antibody.Single-step Protein A and Protein G avidity purification methods to support bispecific antibody discovery and development.Removing a difficult-to-separate byproduct by Capto L affinity chromatography during the purification of a WuXiBody-based bispecific antibody.Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography.Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody.Immunoglobulin-binding protein-based affinity chromatography in bispecific antibody purification: Functions beyond product capture.Comparing the relative robustness of byproduct removal by wash and by elution in column chromatography for the purification of a bispecific antibody.Having the majority of half antibody byproduct removed at this early stage is a big advantage as it improves the overall robustness of the downstream process. In this study, we demonstrated that adding salt additive to Protein A mobile phase can significantly improve resolution between half antibody and the intact bsAb, allowing most of the half antibody impurity in the load to be removed by this capture step. Nevertheless, separation between these two species is far from complete under this condition. Indeed, Protein A chromatography provides certain resolution between half antibody and the intact bsAb under linear pH gradient elution. As half antibody contains only one Fc-domain, it binds Protein A resin weaker than the target bsAb, which contains the full Fc-region. N2 - Half antibody (one half of an antibody which comprises a heavy chain and a light chain) is a common byproduct in bispecific antibody (bsAb) production and in many cases it represents the major product-related impurity. T1 - Removing half antibody byproduct by Protein A chromatography during the purification of a bispecific antibody. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |